$45.35
$74.37
Product Description Pseudoviral Neutralization Assay Kit Utilizing Our NEW Hybrid Alphavirus Pseudovirus Technology. Now with ready-to-use cells! No need to culture cells or CO2 incubators. Cells come pre-counted so just thaw and go. Virongy’s Pseudoviral Neutralization Assay Kit is superior due to the implementation of our newly developed alpha pseudovirus. Alpha pseudoviruses are a newly developed hybrid virus-like particle (VLP) that encapsulates an alphavirus-derived RNA genome for rapid expression of reporter genes (luciferase or GFP) in target cells (Hetrick et al., 2022). Different from commonly used S protein pseudotyped lenti- or vesicular stomatitis virus (VSV)-pseudoviruses, alpha pseudoviruses are assembled with all of the structural proteins of the target virus. These particles contain a reporter genome derived from an alphavirus-based vector for rapid (4 hours) and robust expression of reporter genes. Alpha pseudoviruses represent a major technological advancement in the development of pseudoviruses and serve as a platform for rapid and robust quantification of neutralization assays, viral mutants, and antiviral drugs (Dabbagh et al., 2021; He et al., 2021). Virongy offers pre-assembled reporter pseudovirions and variants. We also help customers to perform antiviral drug screening and quantification of neutralizing antibodies. For more information, please contact us by email:
[email protected] Alpha pseudovirus major advances A simple protocol that is easier than an ELISA-based method Higher Accuracy than a traditional ELISA-based neutralization assay Faster speed compared to other pseudoviruses– (Maximum reporter expression between 12-18 hours) More robust reporter signal – Alpha pseudoviruses take advantage of an alphavirus vector’s rapid and robust gene expression capacity for reporter expression. High fidelity in particle structure – Alpha pseudoviruses contain all of the virus structural proteins but no structural proteins from other viruses. A strong correlation with the live virus in neutralizing antibody assays. Want to read more on the Alpha pseudovirus technology? Click Here
